Next Generation Sequencing
Get Result As Fast As 3 Days
Next generation sequencing is a powerful tool that enables high-throughput sequencing of DNA and RNA, providing insights into genetic profiles, gene expression, microbiome analysis, gene editing validation and more. General workflow includes the following steps:
Recommendations For Read-Based Applications
| Application | Recommand Reads Per Sample (M) |
|---|---|
| Amplicon Sequencing | 0.01 - 0.04 |
| 16S Sequencing | 0.1 - 0.5 |
| Bulk TCR/BCR Sequencing | 1 - 5 |
| Ultra-low input buck RNA-Seq | 5 - 10 |
| Buck small RNA-Seq | 10 - 30 |
| Buck mRNA Sequencing | 20 - 30 |
| Total RNA with rRNA depletion | 20 - 50 |
| Metagenomic non-16S Sequencing | 20 - 50 |
| CHIP-Seq | 20 - 50 |
| Single Cell NGS (per 10000 cell) | 20 - 100 |
| ATAC-Seq | 50 - 100 |
Recommendations For Coverage-Based Applications
| Application | Recommend Coverage |
|---|---|
| Regular whole genome sequencing (WGS) | 20x - 30x |
| Deep whole genome sequencing (WGS) | 100x - 200x |
| Whole Exome Sequencing (WES) | 100x - 200x |
| Targeted Sequencing | 500x - 1000x |
All samples for Amplicon Express service is running every Wedsday and Friday. Submit your samples on time to get the fastest turnaround time, at the pricing at just $60 per sample.
We accept a wide range of starting materials,including tissue, cell, plasmid and gDNA, for amplicon sequencing.Depending on the sample type, we can assist withnucleic acid extraction, PCR and indexing to ensure the highest quality results. Our flexible sequencing options allow for fast turnaround times, with results available in as fast as 5 business days.
Sample Preparation Instructions
Standard Sample Type: Mouse tissue, Cell pellets, Bacteria culture, Plasmid DNA, genomic DNA
Standard Volume: 20 ul DNA at 15-50 ng/ul, or 250,000 cells, or 1 gram tissue or bacteria culture.
Standard Read Length: 250bp pair-end
Other Sample Type and Volume: Please inquire us for a customized service.
Primers: Please provide at least 20uL of PCR primers at 10 pmol, or submit the PCR primer sequence, and we can synthesize it for you.
Sample Container: PCR tubes, strip tubes,PCR plate or Eppendorf tube
Reads: At least 10,000 reads per sample
Price and Turnaround Time
| Sample Type | DNA Extraction | RNA Extraction | Reverse Transcription | PCR/Sequencing (>10,000 Reads) | Additional Depth | Turnaround |
|---|---|---|---|---|---|---|
| Tissue Cell Bacterial Culture |
$50 | $60 | - | $60 | $100 per additional 100,000 reads | 7 business days |
| gDNA/Plasmid | - | - | - | $60 | $100 per additional 100,000 reads | 3 business days |
| RNA | - | - | $50 | - | - | - |
| Other | Please Inquire ngs@quiantarabio.com | |||||
Order
Please email ngs@quintarabio.com to order.
Overview
Looking to study gene expression profiles or identify non-coding RNA associated with your disease model in just one week? Try our RNA Express service. We guarantee delivery of mRNA (poly-A selection) or total RNA (rRNA depletion) sequencing data within 7 business days. Need data analysis and publication-ready figures? Our experts are here to help you generate high-quality figures suitable for top-tier publications.
Sample Preparation Instructions
Standard Sample Type: Mouse tissue, Cell pellets, Purified RNA
Standard Volume: 50 ul RNA at 100 ng/ul with RIN > 7, 250,000 cells, or 1 gram tissue .
Standard Read Length: 75bp pair-end
Other Sample Type and Volume: Please inquire us for a customized service.
Sample Container: PCR tubes, strip tubes, PCR plate or Eppendorf tube
Reads: 20M-30M reads per sample
Price and Turnaround Time
Starting at $159, with raw NGS data delivered within 7 days and data analysis available in as fast as an additional 7 days
Order
Please email ngs@quintarabio.com to order.
Our 10x Single Cell Sequencing service provides a detailed view of cellular diversity by analyzing gene expression at the single-cell level. This cutting-edge technology enables the identification of distinct cell populations, understanding of cellular heterogeneity, and discovery of novel cell types within complex tissues. With high-resolution data, our service is ideal for applications in immunology, oncology, developmental biology, and more. From sample preparation to data analysis, we offer end-to-end solutions to help you uncover unique biological insights with confidence.
Support Chromium Single Cell Gene Expression v4 and Single Cell Immune Profiling v3
For more details please Send Message to ngs@quintarabio.com.
Overview
Our Genomic DNA sequencing service is ideal for whole-genome sequencing of both large genomes, such as human and mouse, and small genomes, like bacteria and viruses. Receive your FASTQ files in as fast as 5 business days. If you need data analysis, we offer complete solutions including variant detection and genomic profiling, ensuring reliable results with rapid turnaround times. Our end-to-end service covers everything from DNA extraction and library preparation to sequencing and data analysis.
Sample Preparation Instructions
Standard Sample Type: Mouse tissue, Cell pellets, Bactieria culture, Plasmid DNA, genomic DNA.
Standard Volume: 20 ul RNA at 15-50 ng/ul, 250,000 cells, 1 gram tissue or bacteria culture.
Standard Read Length: 300bp pair-end
Other Sample Type and Volume: Please inquire us for a customized service.
Sample Container: PCR tubes, strip tubes, PCR plate or Eppendorf tube
Coverage: standard 30x, ultra-deep 300x
Price and Turnaround Time
Starting at $200, with raw NGS data delivered within 7 days and data analysis available in as fast as an additional 7 days. Additional cost for ultra-deep coverager.
Order
Please email ngs@quintarabio.com to order.
Our Nanopore and PacBio sequencing services offer long-read sequencing solutions that provide unparalleled insights into complex genomes, transcriptomes, and epigenomes. Ideal for applications such as de novo genome assembly, structural variant detection, full-length transcript profiling, and microbial community analysis, these technologies deliver highly accurate and comprehensive results. With rapid turnaround times and flexible data analysis options, our service is designed to meet the needs of researchers seeking in-depth genomic insights.
For more details please Send Message to ngs@quintarabio.com.